In RNA gel-blot analysis 35S-ATR1 displayed elevated accumulation of ATR1 and Trp gene transcripts including two Trp synthesis genes; ASA1 and TSB1 and three Trp secondary metabolism genes CYP79B2; CYP79B3; and CYP83B1. ASA1 encodes the catalytic alpha-subunit of anthranilate synthase the first committed enzyme of the Trp pathway and TSB1 encodes the Trp synthase beta-subunit activity that converts indole to Trp (Celenza et al. 2005)