Individual glucosinolates in various organs of the Columbia ecotype of A. thaliana during development with special emphasis on changes occurring during seedling germination and leaf development. The 16 most abundant glucosinolates showed divergent patterns of change during this time course. During the first two days after planting; there was a general increase in all glucosinolates causing total concentration to rise to almost 1.60 nmol (seedling); approximately 3.3% of dry weight. After this point; specific increases were observed for glucosinolates associated with vegetative tissues. Increases of at least 6-fold were detected for 4MSOB; 5-methylsulfinylpentyl-; 1MOI3M and 4-methoxyindol-3-ylmethyl- glucosinolate; while 6 methylsulfinylhexyl-; 7-methylsulfinylheptyl-glucosinolate and I3M increased about 2-fold (Brown et al. 2003)