Biosynthesis of 1-methoxyindol-3-ylmethyl GSL (1MOI3M) from I3M involves the predicted instable intermediate 1-hydroxyindol-3-ylmethyl GSL (1OHI3M) and that IGMT5; a gene with moderate similarity to previously characterized IGMTs encodes the methyltransferase that is responsible for the conversion of 1OHI3M to 1MOI3M. Disruption of IGMT5 function increases resistance against the root-knot nematode Meloidogyne javanica and suggests a potential role for the 1-IG-modification pathway in Arabidopsis below-ground defense (Pfalz et al. 2016)