Genetic mapping and analysis of T-DNA insertions in candidate genes identified BZO1 (At1g65880) which encodes an enzyme with benzoyl-CoA ligase activity as being required for the accumulation of benzoyloxyGSLs. Long-chain aliphatic GSLs are elevated in bzo1 mutants suggesting substrate competition for the common short-chain aliphatic GSL precursors. Whereas bzo1 mutations have seed-specific effects on benzoyloxyGSL accumulation the relative abundance of 3-benzoyloxypropyl- and 4-benzoyloxy-butylGSLs depends on the maternal genotype (Kliebenstein et al. 2007)